Human Diploid Cell Transformation by DNA Extracted from the Tumor Virus SV40

Science  17 Oct 1969:
Vol. 166, Issue 3903, pp. 390-391
DOI: 10.1126/science.166.3903.390


The DNA isolated from simian virus 40 (SV40) can transform human fibroblast cells in tissue culture. Clonal lines of DNA-transformed human cells have been obtained that have properties characteristic of cells transformed by whole virus. They all contain SV40 T-antigen, and infectious virus can be recovered by cocultivation. This is the first demonstration of a permanent genetic alteration produced in human cells by purified DNA.