Nosema cuniculi: In vitro Isolation

Science  24 Oct 1969:
Vol. 166, Issue 3904, pp. 516-517
DOI: 10.1126/science.166.3904.516


A mammalian microsporidian was propagated for the first time in vitro with rabbit choroid plexus monolayer cultures. Tentative classification as Nosema cuniculi (Levaditi, 1923) was established by morphologic and tinctorial characteristics and by extrusion of sintgle polar filaments with attached sporoplasin from living spores. The method permits propagation of large numbers of organisms in putre cultutre.