Plus and Minus Single-Stranded DNA Separately Encapsidated in Adeno-Associated Satellite Virions

Science  05 Dec 1969:
Vol. 166, Issue 3910, pp. 1280-1282
DOI: 10.1126/science.166.3910.1280


Based on physical and chemical determinations, the mnolecular weight of the type 4 adeno-satellite virus is 5.4 X 106 daltons, and the virion contains 1.4 X 106 daltons of DNA. Denaturation and renaturation studies indicate that the viral genome is a single-stranded DNA molecule and that each virion contains either a minus or a plus strand. Upon extraction, the minus and plus strands unite to form double-stranded DNA molecules with no obvious excess of unpaired strands.