Reports

Codon Recognition by Enzymatically Mischarged Valine Transfer Ribonucleic Acid

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Science  26 Dec 1969:
Vol. 166, Issue 3913, pp. 1635-1637
DOI: 10.1126/science.166.3913.1635

Abstract

Direct evidence for the adaptor hypothesis has been obtained by examining the codon recognition of a purified Escherichia coli valine transfer ribonucleic acid which was enzymatically mischarged with phenylalanine labeled with carbn-14 by reaction with purified phenylalanyl-transfer ribonucleic acid synthetase from Neurospora crassa. The mischarged transfer ribonucleic acid recognized the valine codons but failed to recognize the phenylalanine codon when tested in trinucleotide-directed ribosomal binding assay.