Animal Cells: Noncorrelation of Length of G1 Phase with Size after Mitosis

Science  02 Jan 1970:
Vol. 167, Issue 3914, pp. 80-82
DOI: 10.1126/science.167.3914.80


The interval between mitosis and initiation of DNA synthesis (G1) varied over a fourfold range for Chinese hamster ovary cells, an established line. This was not because of size differences. Synchlronous cells of different sizes began DNA synthesis at similar times after mitosis. A novel technique of centrifugation for separating cells according to size is described.