Growth in vitro of Cells from Hyperplastic Nodules of Liver Induced by 2-Fluorenylacetamide or Aflatoxin B1

Science  16 Jan 1970:
Vol. 167, Issue 3916, pp. 285-287
DOI: 10.1126/science.167.3916.285


Cell suspensions obtained from hyperplastic nodules induced in rat liver by either of the two hepatic carcinogens, 2-fluorenylacetamide or aflatoxin B1, show growth when cultured in vitro. No growth of cells from liver adjacent to the hyperplastic nodules or from liver of control rats has been obtained so far under comparable conditions. Hepatocarcinoma cells induced by 2-fluorenylacetamide grow readily in vitro but behave differently. These findings suggest that some nonmalignant cells capable of growth in vitro arise during liver carcinogenesis prior to the appearance of unequivocal cancer. Cultures of such cells may offer new avenues for the study of liver carcinogenesis.