5S RNA Synthesized by Escherichia coli in Presence of Chloramphenicol: Different 5'-Terminal Sequences

Science  23 Jan 1970:
Vol. 167, Issue 3917, pp. 382-384
DOI: 10.1126/science.167.3917.382


Escherichia coli cells, grown in the presence of chloramphenicol, synthesize a low molecular weight RNA (CM-5S RNA) not bound to ribosomes which is similar to ribosomal 5S RNA. Oligonucleotide patterns derived from ribonuclease digests of 5S RNA and of CM-5S RNA are indistinguishable except that the 5'-terminal oligonucleotides differ. Whereas the nucleotide sequence of the 5'-terminus of normal 5S RNA is pUpG-, there are three alternate sequences of the 5'-terminus of CM-5S RNA: pUpUpG-, pUpUpUpG-, and pApUpUpUpG-.