Horseshoe Crab Lactate Dehydrogenase: Tissue Distribution and Molecular Weight

Science  13 Feb 1970:
Vol. 167, Issue 3920, pp. 994-996
DOI: 10.1126/science.167.3920.994


Lactate dehydrogenase from Xiphosura (Limulus) polyphemus i D(-)-lactate specific. It does not use L(+)-lactate, α-hydroxybutyrate, α-hydroxyvalerate, or α-hydroxyisocaproate as substrate. In most tissues lactate dehydrogenase is composed of five isozymes with a molecular weight of 140,000 for each, as judged by gel-filtration chromatography. This suggests that the isozyme, are tetramers comprised of varying amounts of two physicochemically distinc subunits.