Oligodeoxyribonucleotides: Chemical Synthesis in Anhydrous Base

Science  27 Feb 1970:
Vol. 167, Issue 3922, pp. 1266-1268
DOI: 10.1126/science.167.3922.1266


The sugar hydroxyl of a methoxytrityldeoxyribonucleoside reacts with a methoxytrityldeoxyribonucleoside phosphorofluoridate in dimethylformamide and potassium tertiary butoxide to yield the protected dinucleoside monophosphate. The reaction is fast and specific, and is used in a stepwise synthesis to prepare trinucleoside diphosphate and tetranucleoside triphosphate.