Heme Requirement for Reproduction of a Free-Living Nematode

Science  03 Apr 1970:
Vol. 168, Issue 3927, pp. 143-144
DOI: 10.1126/science.168.3927.143


The free-living hermaphroditic nematode Caernorhabditis briggsae has a nutritional requirement for heme. The organism can be subcultured repeatedly in a chemically defined axenic medium that contains autoclaved bacterial cells (Escherichia coli) and sterols if a hemeprotein-containing fraction from liver is present. Pure myoglobin, hemoglobin, cytochrome c, and hemin, respectively, can substitute effectively for the liver fraction.