Malaria Resistance: Artificial Induction with a Partially Purified Plasmodial Fraction

Science  29 May 1970:
Vol. 168, Issue 3935, pp. 1117-1118
DOI: 10.1126/science.168.3935.1117


Plasmodium berghei were released from mouse erythrocytes by passage through a French pressure cell. The released organisms were washed and disintegrated; the soluble portion was chromatographed on a Sephadex (G-200) column. The void-volume eluate contained an erythrocyte-free plasmodial fraction which behaved as a vaccine, preventing parasitemia, anemia, and death in mice subsequently challenged with living Plasmodium berghei.