Automated Continuous Culture of Mammalian Cells in Suspension

Science  10 Jul 1970:
Vol. 169, Issue 3941, pp. 204-205
DOI: 10.1126/science.169.3941.204


A system has been developed for the continuous culture of mammalian cells in suspension. The system maintains constant cell concentrations (monitored as the degree of light scattering) over a wide range of previously selected values by automatic additions of known amounts of medium and simultaneous withdrawals of equal volumes of cell suspension.