Puffing and Histone Acetylation in Polytene Chromosomes

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Science  24 Jul 1970:
Vol. 169, Issue 3943, pp. 373-374
DOI: 10.1126/science.169.3943.373


Fixation with picric acid or formaldehyde retains incorporated [3H]acetate which is lost after fixation with ethanol and acetic acid. Unlike [3H]uridine, [3H]acetate is diffusely incorporated into polytene chromosomes, and not preferentially into existing or newly induced puffs. It is suggested that puff formation does not include an acetylation of histones.