Antibody to Nuclear Material Eluted from Isolated Spleen Vessels in Systemic Lupus Erythematosus

Science  30 Oct 1970:
Vol. 170, Issue 3957, pp. 550-551
DOI: 10.1126/science.170.3957.550


Splenic blood vessels were isolated from surrounding cells by treatment with trypsin and the use of ultrasound. This procedure retained vascular-bound immunoglobulins which were recovered by acid elution of vessels isolated from a patient with systemic lupus erythematosus. These eluted immunoglobulins reacted with material from cell nuclei as demonstrated by immunofluorescence.