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Radioreceptor Assay of Adrenocorticotropic Hormone: New Approach to Assay of Polypeptide Hormones in Plasma

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Science  06 Nov 1970:
Vol. 170, Issue 3958, pp. 633-635
DOI: 10.1126/science.170.3958.633

Abstract

Biologically active iodine-125-labeled adrenocorticotropic hormone (ACTH) binds specifically to ACTH receptors extracted from adrenals. Unlabeled ACTH at 1 picogram per milliliter significantly displaces labeled ACTH from these receptors. This system, which appears to be applicable to all polypeptide hormones, provides a rapid and sensitive method for measurements of biologically active ACTH in dilute whole plasma.