Stimulation in vitro with Protein Carrier of Antibodies against a Hapten

Science  19 Mar 1971:
Vol. 171, Issue 3976, pp. 1165-1167
DOI: 10.1126/science.171.3976.1165


Rabbits were immunized both with lysozyme and with dinitrophenylated bovine serum albumin. No antibodies against the dinitrophenyl hapten were produced when cell suspensions of their spleens were exposed in vitro to dinitro-phenyl-ovalbumin, whereas a positive response was obtained with dinitrophenyl-lysozyme. Moreover, when spleen cells from rabbits previously immunized with dinitrophenyl-bovine serum albumin were exposed in vitro to bovine serum albumin alone, they produced antibodies against the dinitrophenyl hapten.