New Class of Purine Mutants of Chinese Hamster Ovary Cells

Science  09 Apr 1971:
Vol. 172, Issue 3979, pp. 162-163
DOI: 10.1126/science.172.3979.162


Mutants of Chinese hamster ovary cells (CHO/Pro-) were isolated after mutagenesis with N-methylnitrosoguanidine and selection by the bromodeoxyuridine technique. Six of these were mutants classified as purine-requiring. The metabolic block appears to be early in the purine biosynthetic pathway. The mutants do not appear to be genetically identical.