Preparation of Highly Labeled [32P]Nucleic Acids from Yeast; Isolation of "Denaturable" Leucine Acceptor Transfer RNA

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Science  23 Apr 1971:
Vol. 172, Issue 3981, pp. 384-385
DOI: 10.1126/science.172.3981.384


Culture conditions were developed that permit efficient incorporation of [32P]phosphate into nucleic acids of baker's yeast. RNA sufficiently labeled for sequence determination was obtained in this way. Pure "denaturable" leucine acceptor transfer RNA (tRNALeu3) was isolated for this purpose.