Nucleotide Sequence of the "Denaturable" Leucine Transfer RNA from Yeast

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Science  23 Apr 1971:
Vol. 172, Issue 3981, pp. 385-387
DOI: 10.1126/science.172.3981.385


The nucleotide sequence of " denaturable"leucine acceptor transfer RNA (tRNALeu3) from baker's yeast was determined on 32P-labeled material. The molecule is 85 nucleotides long and can be folded into the "cloverleaf" model for secondary structure. The basis on which the sequence was deduced from the products of complete enzymatic digestion, prior to its unambiguous determination, is presented.