Cell Surface Antigens: Serial Sectioning of Single Cells as an Approach to Topographical Analysis

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Science  30 Apr 1971:
Vol. 172, Issue 3982, pp. 472-474
DOI: 10.1126/science.172.3982.472


The topographical distribution of H-2 antigens on the surfaces of C57BL/6 mouse thymocytes and lymph-node lymphocytes was investigated by a new technique. Single cells were reacted with visually labeled antibody directed against H-2 antigen. Each cell was processed for electron microscopy individually and was serially sectioned. Models constructed from serial electron micrographs provided detailed views of the entire cell surface and showed that H-2 antigen occurs on thymocytes in small isolated regions and on lymphocytes in large interconnected regions.