Actinomycin D: Renewed RNA Synthesis after Removal from Mammalian Cells

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Science  07 May 1971:
Vol. 172, Issue 3983, pp. 577-579
DOI: 10.1126/science.172.3983.577


Suppressed RNA synthesis exceeded control rates 2 hours after removal of actinomycin from serial lines of green monkey kidney cells in exponentially dividing monolayer cultures. Enhanced synthesis was directly related to the dose of actinomycin, and release from inhibition was temperature dependent. A broad range of RNA sizes was made after removal of drug, but cytoplasmic and nucleolar sedimentation profiles were abnormal for at least 20 hours.