Synthesis of 5S and 4S RNA in Metaphase-Arrested HeLa Cells

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Science  28 May 1971:
Vol. 172, Issue 3986, pp. 947-949
DOI: 10.1126/science.172.3986.947


The continued synthesis of both 5S and 4S RNA in metaphase-arrested HeLa cells is demonstrated; 5S RNA is apparently synthesized at approximately 74 percent of the interphase rate, while 4S RNA is synthesized at approximately one-third the rate. The ratio of uridine incorporation to RNA methylation is used to correct for the alteration in the specific activity of the pyrimidine pool during metaphase arrest.