Salt Extraction of Soluble HL-A Antigens

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Science  11 Jun 1971:
Vol. 172, Issue 3988, pp. 1134-1136
DOI: 10.1126/science.172.3988.1134


Extraction of cultured human lymphoid cells with hypertonic salt solutions (3 molar potassium chloride) resulted in high recoveries of membrane-associated histocompatibility (HL-A) antigens in soluble form with potent activity and marked immunologic specificity. The active principle was purified by preparative acrylamide-gel electrophoresis. Application of the hypertonic salt extraction method is now yielding sufficient HL-A antigen to begin the elucidation of the molecular basis of transplantation individuality.