Production of Hemadsorption-Negative Areas by Serums Containing Australia Antigen

Science  18 Jun 1971:
Vol. 172, Issue 3989, pp. 1265-1267
DOI: 10.1126/science.172.3989.1265


Exposure of human Wi-38 cells to human serums containing Australia antigen, and presumably serum hepatitis virus, renders the cells refractory to infection by Newcastle disease virus as detected by the hemadsorption-negative plaque test for intrinsic interference. Induction of the Newcastle disease virus refractory state could be passed in cell culture with up to a 1 : 100,000 dilution of material obtained from cells "infected" with serums containing Australia antigen after filtration (0.45-µm pores) and heating to 60°C for 1 hour. Human antiserums to the Australia antigen prevented induction of the Newcastle disease virus refractory state.