Trout Leukocytes: Growth in Oxygenated Cultures

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Science  16 Jul 1971:
Vol. 173, Issue 3993, pp. 246-247
DOI: 10.1126/science.173.3993.246


A reproducible method of culturing leukocytes from rainbow trout (Salmo gairdneri) has been found. Using an atmosphere of 100 percent oxygen, we stimulated peripheral blood leukocytes to divide at 19°C. Cultured cells were used for a karyological study; they had a modal chromosome number of 60 and a constant arm number of 104, a value previously reported for other tissues in these fish.