Acetylcholine Binding to Torpedo Electroplax: Relationship to Acetylcholine Receptors

Science  23 Jul 1971:
Vol. 173, Issue 3994, pp. 338-340
DOI: 10.1126/science.173.3994.338


Binding of [3H]acetylcholine to a particulate fraction of Torpedo electroplax was measured by equilibrium dialysis. Two high-affinity sites present on phospholipoproteins bound acetylcholine reversibly, and binding was blocked by nicotinic drugs. Characteristics of this binding suggest that these phospholipoproteins may be acetylcholine receptors.