Cell Dissociation: Univalent Antibodies as a Possible Alternative to Proteolytic Enzymes

See allHide authors and affiliations

Science  20 Aug 1971:
Vol. 173, Issue 3998, pp. 742-743
DOI: 10.1126/science.173.3998.742


Univalent antibody fragments directed against special membrane antigens dissociate multicellular bodies of the cellular slime mold Dictyostelium discoideum completely into single cells. This provides a gentle method for cell dissociation and demonstrates that a nonenzyme protein can disintegrate a tissue