Ascorbic Acid: A Culture Requirement for Colony Formation by Mouse Plasmacytoma Cells

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Science  12 Nov 1971:
Vol. 174, Issue 4010, pp. 720-722
DOI: 10.1126/science.174.4010.720


Plasmacytoma stem cells explanted from mice formed colonies in culture only in the presence of L-ascorbic acid; this vitamin was not needed for the formation of granulocytic colonies. Isomers of L-ascorbic acid with less antiscorbutic activity also promoted plasmacytoma colony formation, but less effectively. Other redox compounds without antiscorbutic activity and an antioxidant were ineffective.