Human Leukemic Cells: In vitro Growth of Colonies Containing the Philadelphia (Ph1) Chromosome

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Science  10 Dec 1971:
Vol. 174, Issue 4014, pp. 1134-1136
DOI: 10.1126/science.174.4014.1134


Human leukemic cells with a marker (Philadelphia; Ph1) chromosome gave rise to granulocytic and mononuclear cell colonies when grown in vitro. All metaphases from a single colony were either Ph1 positive or Ph1 negative. No colonies contained a mixed cell population. This suggests that leukemic and normal cells exist simultaneously and that in vitro colonies are clonal in origin.