Glycoproteins: Isolation from Cell Membranes with Lithium Diiodosalicylate

Science  17 Dec 1971:
Vol. 174, Issue 4015, pp. 1247-1248
DOI: 10.1126/science.174.4015.1247


A glycoprotein has been extracted in water-soluble form from human red cell membranes with lithium diiodosalicylate. After extraction of the membranes and phosphocellulose chromatography a homogeneous preparation is obtained which was 60 percent carbohydrate and 40 percent protein (by weight). The preparation contains AB and MN blood group antigens, receptors for influenza virus, and various phytohemagglutinins.