Mechanism of Gonadotropin Action in Amphibia: Involvement of Mitochondria

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Science  07 Jan 1972:
Vol. 175, Issue 4017, pp. 67-68
DOI: 10.1126/science.175.4017.67


Luteinizing hormone (a pituitary gonadotropic hormone) stimulates Δ5-3β- hydroxysteroid dehydrogenase activity in the microsomal fraction of frog testes when incubated together with the mitochondria; incubation together with the nuclei instead of the mitochondria does not result in increased, Δ5-3β-hydroxysteroid dehydrogenase activity. The increase is not, induced by adenosine triphosphate, it appears to be hormone-specific, and it is sensitive to puromycin and actinomycin D. These data suggest that the mitochondrial DNA may be involved in mediating the action of luteinizing hormone in amphibian steroidogenesis.