Cytochalasin B: Does It Affect Actin-Like Filaments?

Science  18 Feb 1972:
Vol. 175, Issue 4023, pp. 774-776
DOI: 10.1126/science.175.4023.774


An in vitro system was used to test the purported action of cytochalasin B. At concentrations 100 times those used for experiments in vivo, cytochalasin B did not cause the breakdown of F-actin, did not inhibit the transformation of G-actin to F-actin, did not inhibit the binding of heavy meromyosin to F-actin, and did not inhibit the adenosine triphosphate-induced release of heavy meromyosin from F-actin.