Restoration of Antibody-Forming Capacity in Cultures of Nonadherent Spleen Cells by Mercaptoethanol

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Science  07 Apr 1972:
Vol. 176, Issue 4030, pp. 60-61
DOI: 10.1126/science.176.4030.60


Populations of mouse spleen cells, without those cells that adhere to glass or plastic, exhibit little or no capacity for formation of antibody to sheep erythrocytes in the Mishell-Dutton culture system. When 5 x 10-5M mercaptoethanol is added to the culture medium, the antibody-forming capacity of these nonadherent spleen cells is restored to that of unfractionated spleen cells.