Radioautography of the Optic Tectum of the Goldfish after Intraocular Injection of [3H]Proline

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Science  28 Apr 1972:
Vol. 176, Issue 4033, pp. 407-410
DOI: 10.1126/science.176.4033.407


Radioautography of the optic tectum of the goldfish, performed after injection of [3H]proline into the contralateral eye, effectively resolves several distinct layers of retinal synapses. Silver grains are found unilaterally over nerve tracts containing efferent fibers from the tectum, a result that suggests intercellular migration of labeled molecules. The low background and high specific grain density obtained with [3H]proline radioautography indicate the usefulness of this technique for the elucidation of neuroanatomical connections in the visual system.