Biosynthesis of C4 (Fourth Component of Complement) by Hybrids of C4-Deficient Guinea Pig Cells and HeLa Cells

See allHide authors and affiliations

Science  02 Jun 1972:
Vol. 176, Issue 4038, pp. 1029-1031
DOI: 10.1126/science.176.4038.1029


Peritoneal exudate cells from guinea pigs homozygous for a genetic deficiency of the fourth component of complement (C4) were fused in vitro with a cell line of human origin (HeLa). The resulting hybrid cells, derived from cell lines each incapable of C4 synthesis by themselves, synthesized functionally active human C4.