Collagenase: Localization in Polymorphonuclear Leukocyte Granules in the Rabbit

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Science  07 Jul 1972:
Vol. 177, Issue 4043, pp. 64-65
DOI: 10.1126/science.177.4043.64


Polymorphonuclear leukocyte granules were submitted to zonal fractionation through a discontinuous sucrose gradient. Azurophilic and specific granules were enzymatically characterized by peroxidase and alkaline phosphatase activity, respectively. The enzymes formed modal distributions like those reported by others. Collagenase activity was consistently associated with the specific granules containing alkaline phosphatase.