Terminal Saccharides on Sperm Plasma Membranes: Identification by Specific Agglutinins

Science  21 Jul 1972:
Vol. 177, Issue 4045, pp. 276-279
DOI: 10.1126/science.177.4045.276


Six specific agglutinins were used to identify the terminal sugar residues in the surface oligosaccharides of rabbit and hamster spermatozoa by specific agglutination. Species differences in epididymal sperm were found in the terminal residues, resembling α-D-mannose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine. Species similarities were found in terminal residues, resembling L-fucose and N-acetylneuraminic acid. When ejaculated rabbit sperm were compared to epididyimal sperm, the latter were more agglutinable with a specific agglutinin recognizing N-acetyl-D-glucosamine.