Fluorescent Modification of Adenosine 3',5'-Monophosphate: Spectroscopic Properties and Activity in Enzyme Systems

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Science  21 Jul 1972:
Vol. 177, Issue 4045, pp. 279-280
DOI: 10.1126/science.177.4045.279


The synthesis of a highly fluorescent analog of adenosine 3',5'-monophosphate, namely, 1,N6-ethenoadenosine 3',5'-monophosphate, has provided a powerful probe for systems involving adenosine 3',5'-monophosphate. The potential utility of this analog is indicated by its long fluorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3',5'-monophosphate.