Adenosine 3',5'-Monophosphate Is Localized in Cerebellar Neurons: Immunofluorescence Evidence

Science  04 Aug 1972:
Vol. 177, Issue 4047, pp. 436-438
DOI: 10.1126/science.177.4047.436


Adenosine 3',5'-monophosphate is localized in specific cerebellar neurons, as shown by fluorescence immunocytochemistry with a specific rabbit immunoglobulin. Positive staining is exhibited by Purkinje neurons and granule cells. The increase in concentration of cyclic adenosine monophosphate in the cerebellum, which is known to follow decapitation, is represented by greatly increased fluorescence of Purkinje neurons only. These immunofluorescence data provide the first evidence for localization of cyclic adenosine monophosphate in specific neurons and may permit further exploration into the role of this cyclic nucleotide in neuronal function.