Acrosomal Proteinase and Proteinase Inhibitor of Human Spermatozoa

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Science  25 Aug 1972:
Vol. 177, Issue 4050, pp. 702-703
DOI: 10.1126/science.177.4050.702


The acrosomal proteinase of human spermatozoa was characterized and differs from other human proteinases. The enzyme has optimal activity at pH 8.0, is inactive below pH 5.0 or above pH 10.5, requires calcium for maximum activity, hydrolyzes fibrinogen, gelatin, and benzoylarginine ethyl ester, and is inhibited by various synthetic and natural proteinase inhibitors. The molecular weight was estimated to be 30,000. Human spermatozoa also possess a proteinase inhibitor that is similar to one of the proteinase inhibitors from human seminal plasma.