Aryl Hydrocarbon Hydroxylase Induction in Human Leukocytes

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Science  20 Oct 1972:
Vol. 178, Issue 4058, pp. 315-316
DOI: 10.1126/science.178.4058.315


A method for determining aryl hydrocarbon hydroxylase induction in human leukocytes is described. Leukocytes from healthy volunteers were cultured in the presence of phytohemagglutinin, a mitogen. Addition of 3-methylcholanthrene to 72-hour cultures induced a fourfold increase in aryl hydroxylase activity. In the absence of a mitogenic agent, 3-methylcholanthrene stimulation of increased enzymatic activity did not occur.