Primary Structure of Neocarzinostatin, an Antitumor Protein

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Science  24 Nov 1972:
Vol. 178, Issue 4063, pp. 875-876
DOI: 10.1126/science.178.4063.875


The antitumor protein neocarzinostatin is an acidic single-chain molecule, cross-linked by two disulfide bridges, and consists of 109 amino acid residues. Complete disulfide bond reduction and S-carboxymethylation was achieved in liquid ammonia. Sequence determination of five tryptic fragments led to the proposed primary structure.