Reports

Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism

Science  15 Dec 1972:
Vol. 178, Issue 4066, pp. 1209-1210
DOI: 10.1126/science.178.4066.1209

Abstract

This new enzymatic method disperses rabbit lung into morphologically intact, viable individual cells. The scattered cells constitute more than 50 percent of the original tissue. At least 90 percent of the cells exclude trypan blue from the nucleus. The dispersed lung cells consumed 6.2 microliters of oxygen per hour per milligram, dry weight. They incorporated [1-14C]palmitate into lecithin.

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