Reports

Translation of Bacteriophage Qβ Messenger RNA in a Murine Krebs 2 Ascites Tumor Cell-Free System

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Science  22 Dec 1972:
Vol. 178, Issue 4067, pp. 1293-1295
DOI: 10.1126/science.178.4067.1293

Abstract

Qβ is a small bacterial virus whose three genes are encoded in a single-stranded molecule of RNA. This RNA serves directly as the Qβ message. Here we describe conditions under which RNA corresponding to the coat cistron of this bacterial virus is translated in a system derived from mammalian cells. Translation of the bacterial virus messenger RNA is less effective than that of mammalian globin messenger RNA, but is somewhat enhanced by mild alkali treatment of the messenger. The synthesized product when subjected to electrophoresis migrates with authentic Qβ coat protein and yields tryptic peptides that correspond to those derived from the Qβ coat protein.