Isolation and Structure Determination of Blepharismin, a Conjugation Initiating Gamone in the Ciliate Blepharisma

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Science  26 Jan 1973:
Vol. 179, Issue 4071, pp. 400-402
DOI: 10.1126/science.179.4071.400


One of the gamones (gamone II) which are effective for the induction of conjugation in Blepharisma intermedium has been isolated in a crystalline form and designated as blepharismin. From the result of chemical and spectroscopic investigations, in which x-ray crystallographic analysis was used as a definitive tool, blepharismin has been found to have the structure of calcium 3-(2'-formylamino-5'-hydroxybenzoyl)lactate.