Noradrenergic Stimulation of Cyclic Adenosine Monophosphate in Rat Purkinje Neurons: An Immunocytochemical Study

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Science  09 Feb 1973:
Vol. 179, Issue 4073, pp. 585-588
DOI: 10.1126/science.179.4073.585


A specific immunofluorescent histochemical method for cyclic adenosine monophosphate was used to study rat cerebellum. After topical treatment with norepinephrine or stimulation of norepinephrine-containing afferents from locus coeruleus, there was a striking increase in the number of Purkinje cells with strong cyclic adenosine monophosphate reactivity. Other putative inhibitory transmitters had no significant effect on staining of Purkinje cells. The results provide the first histochemical support for the hypothesis that cyclic adenosine monophosphate can be generated postsynaptically in central neurons in response to noradrenergic stimuli.