Synthesis of Infective Poliovirus in BSC-1 Monkey Cells Enucleated with Cytochalasin B

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Science  02 Mar 1973:
Vol. 179, Issue 4076, pp. 915-916
DOI: 10.1126/science.179.4076.915


BSC-1 monkey kidney cells were enucleated by two cycles of centrifugation in the presence of cytochalasin B. The resulting populations contained 99.5 percent enucleated cells. After infection, newly synthesized poliovirus was recovered from the enucleated populations. Final virus yield per enucleated cell was about one-fifth the yield per infected untreated cell