Cholesterol Hydroperoxide Formation in Red Cell Membranes and Photohemolysis in Erythropoietic Protoporphyria

Science  16 Mar 1973:
Vol. 179, Issue 4078, pp. 1131-1133
DOI: 10.1126/science.179.4078.1131


3β-Hydroxy-5α-hydroperoxy-Δ6-cholestene is produced in protoporphyrin-containing red blood cell ghosts irradiated with approximately 400-nanometer light in the presence of oxygen. Incorporation of this cholesterol photooxidation product into normal red blood cells leads to increased osmotic fragility and eventual hemolysis. These results may be relevant to photohemolysis associated with erythropoietic protoporphyria.