Cloning of Rat Kangaroo (PTK2) Cells Following Laser Microirradiation of Selected Mitotic Chromosomes

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Science  30 Mar 1973:
Vol. 179, Issue 4080, pp. 1333-1334
DOI: 10.1126/science.179.4080.1333


Mitotic chromosomes of rat kangaroo cells were irradiated with a green argon laser microbeam without prior dye sensitization. Deoxyribonucleic acid-negative chromosome paling was observed. The irradiated cells were isolated and cloned into viable populations.