Radioreceptor Assay for Prolactin and Other Lactogenic Hormones

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Science  01 Jun 1973:
Vol. 180, Issue 4089, pp. 968-971
DOI: 10.1126/science.180.4089.968


A radioreceptor assay with a sensitivity of 5 nanograms per milliliter has been developed for mammalian and avian pituitary prolactin, placental lactogenic hormones, and humnan growth hormone, using a membrane receptor preparation isolated from rabbit mammary glands. Prolactin preparations inhibited the binding of [125I]prolactin to receptors in direct proportion to the biological potency of these preparations. Thus, the radioreceptor assay provides a convenient and simnple assay for hormones which have lactogenic activity.